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KMID : 0364820220580030142
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Korean Journal of Microbiology
2022 Volume.58 No. 3 p.142 ~ p.149
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Surveillance of Burkholderia spp. in Bukit Merah Orang Utan Island (BMOUI), Perak, Malaysia
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Mohamad Nurul Iman
Zakaria Nurul Aili
Dharmalingam Sabapathy
Deris Zakuan Zainy
Yusof Farida Zuraina Mohd
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Abstract
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Melioidosis is an infection caused by Burkholderia pseudomallei and it represents one of the most common infectious diseases in zoo and farm animals giving rise to high mortality, leading to biological diversity loss. We use the bacteriological and biochemical methods such as culture, biochemical test, Gram-staining and oxidase test to examine the presence of B. pseudomallei in 50 soils treated and non-treated with quicklime powder collected from Bukit Merah Orang Utan Island, (BMOUI), Perak, Malaysia.
We observed mixed bacterial colony growth with various colony morphologies. Six of the isolates labelled as P2(25), P5(2), P5(3), P5(5), P6(1), and P6(6)2 showed pinkish purple colour with wrinkled characteristics similar to B. pseudomallei colony morphology after incubation for 48 h. The results showed that all putative soil-isolated organisms were Gram-negative bacteria, positive for oxidase test and negative for catalase test. In the VITEK® ID system and PCR sequencing analysis, no B.
pseudomallei was identified. All six putative organisms were identified as in B. cepacia complex i.e.: B. stagnalis (four cases), B. contaminans (one) and B. multivorans (one). These preliminary findings demonstrate that a few species of B. cepacia group were successfully isolated from the soil of BMOUI using selective media for B. pseudomallei.
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KEYWORD
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Burkholderia sp., conservation, , melioidosis, Orang Utan, soil
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